Determination of 18 Polycyclic Aromatic Hydrocarbons in Vegetable Oil by High Performance Liquid Chromatography with Diodearry Detector and Fluorescence Detector

doi:10.7506/spkx1002-6630-201406030

Abstract

Abstract:

A method has been developed for the simultaneous determination of 18 polycyclic aromatic hydrocarbons in
vegetable oil by gel permeation chromatography (GPC) and high performance liquid chromatography with diodearray
detector and fluorescence detector (HPLC-DAD-FLD). The samples were dissolved in cyclohexane-ethyl acetate (1:1, V/V),
then purified with GPC to remove grease molecules, and separated on a PAH C18 column using acetonitrile and water as the
mobile phase with gradient elution. Subjected to 2-wavelength channel data acquisition setting with fluorescence procedure,
17 kinds of polycyclic aromatic hydrocarbons in oil samples were determined. Data acquisition of acenaphthylene was
achieved by DAD detector. The detection limit of this method was 0.5–4 ng/mL. The average recovery rate in oil was in the
range of 78.65%–103.4% with relative standard deviation (RSD) of less than 5%. This method was satisfactorily used for
the simultaneous detection of 18 kinds of polycyclic aromatic hydrocarbons (PAHs) in edible oil, while meeting the new
detection requirements of Germany standards for these PAHs.

Key words: vegetable oil, polycyclic aromatic hydrocarbons (PAHs), high performance liquid chromatography (HPLC), fluorescence detector

CLC Number:

TS202.3

WANG Feng1, ZHANG Zhi-jie1, LIN Hui1, CANG Yi-peng2,*. Determination of 18 Polycyclic Aromatic Hydrocarbons in Vegetable Oil by High Performance Liquid Chromatography with Diodearry Detector and Fluorescence Detector[J]. FOOD SCIENCE, 2014, 35(6): 142-145.

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