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Extraction and Biochemical Characteristics of Phenoloxidase from Penaeus vannamei

LÜ Yan-fang, WEI Chun-jiao, WANG Jiao, MA Chun-ying, LI Jian-rong   

  1. Food Safety Key Laboratory of Liaoning Province, Engineering and Technology Research Center of Food Preservation, Processing and
    Safety Control of Liaoning Province, College of Chemistry, Chemical Engineering and Food Safety,
    Bohai University, Jinzhou 121013, China
  • Online:2014-04-15 Published:2014-04-18

Abstract:

The extraction of phenoloxidase from Penaeus vannamei with Tris-HCl buffer as a function of three experimentalparameters including extractant concentration, pH and extraction time was optimized using an L9 (33) orthogonal arraydesign. The extracted enzyme was characterized. The highest phenoloxidase activity was obtained after 20 min of extractionwith 0.2 mol/L Tris-HCl buffer at pH 8.0. This enzyme exhibited an optimal pH of 8 and temperature of 40 ℃ for catalyzingthe oxidation of pyrocatechol as a substrate. Kinetic studies obeyed the Michaelis-Menten equation. The Km and Vmax werecalculated to be 0.717 mol/L and 0.130 A/min, respectively.

Key words: Penaeus vannamei, phenoloxidase, orthogonal array design, extraction, kinetics