Recombinant Expression of the Bovine Prochymosin Gene in Food-Grade Lactococcus lactis

doi:10.7506/spkx1002-6630-201407025

Abstract

Abstract:

In this study, we cloned the bovine prochymosin gene pcm into the expression vector pNZ8149 and transformedinto Lactococcus lactis NZ3900. The intracellular chymosin activity attained 0.7 SU/mL in the presence of nisin induction,suggesting that the bovine chymosin gene was successfully expressed in the food-grade nisin controlled gene expressionsystem (NICE). Furthermore, the secretion signal SPusp45 was ligated into pNZ8149 to generate pNZ8149s. RecombinantL. lactis containing pNZ8149s-pcm showed a chymosin activity of 1.2 SU/mL in culture supernatant after induction by 1 ng/mLnisin for 5 h. This result demonstrated that bovine chymosin could be secreted by the expression vector pNZ8149s. Thisstudy provides a feasible method for producing recombinant bovine chymosin in food-grade strains.

Key words: chymosin, Lactococcus lactis, food-grade strain, expression

ZHANG Yan-li, NIE Chun-ming, ZHOU Li-wei, ZHANG Wei, ZHANG Yu-hong, YANG Xiao-hong. Recombinant Expression of the Bovine Prochymosin Gene in Food-Grade Lactococcus lactis[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201407025.

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Recombinant Expression of the Bovine Prochymosin Gene in Food-Grade Lactococcus lactis

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