Abstract:

In this study, various fermentation conditions for the production of the antibacterial peptide Spinigerin α in a 50-Lfermentor, including initial glycerol concentration, feeding methods, methanol/sorbitol ratio, induction temperature, pH andtime were optimized based on the previously established shake flask fermentation conditions. An orthogonal array designinvolving methanol/sorbitol ratio (V/V), induction temperature and pH was used. Results showed that the optimal pilot-scalefermentation conditions were 10 g/L initial glycerol concentration, feeding at variable speeds, 1:1 methanol/sorbitol ratio (V/V)and induction at 24 ℃ and pH 5.0 for 96 h. The yield of Spinigerin α in a 50 L fermentor under the optimized conditions wasincreased by 20% than under shake flask fermentation conditions.

Key words: the antibacterial peptide Spinigerin α, pilot scale, fermentation conditions