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A Real-Time Polymerase Chain Reaction Method to Detect Genetically Modified Wheat Strains B73-6-1, B72-8-11b and B102-1-2

CAO Ji-juan1, XU Jun-yi 1, CAO Dong-mei1, ZHANG Pi-qiao1,2, LUAN Feng-xia3, LIU Yang1   

  1. 1. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China;
    2. College of Life Science, Liaoning Normal University, Dalian 116029, China;
    3. Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin 150001, China
  • Online:2014-04-25 Published:2014-05-13

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Abstract:

Aim: To establish a real-time PCR method for the identification of transgenic wheat strains B73-6-1, B72-8-11b
and B102-1-2. Methods: Specific primers and probes were designed according to the flanking sequences of exogenous
fragments of transgenic wheat and wheat chromosomes. The specificity of the developed method was validated by using
it to detect a variety of other genetically modified crops and non-genetically modified wheat strains. B73-6-1 was used
to prepare 10 content gradients to test the sensitivity. Results: The primers and probes designed showed good detection
specificity for transgenic wheat strains, with its sensitivity reaching 0.01% (m/m). Conclusion: A real-time PCR method for
the identification of three genetically modified wheat strains has been established. This method is of high specificity, high
reproducibility, rapid identification and excellent accuracy for the identification of genetically modified wheat strains B73-6-1,
B72-8-11b and B102-1-2.

Key words: genetically modified wheat, B73-6-1, B72-8-11b, B102-1-2, event specific detection, real-time polymerase chain reaction (PCR)

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