A Real-Time PCR to Detect Horse and Donkey Ingredients in Donkey Hide Glue

doi:10.7506/spkx1002-6630-201408016

FOOD SCIENCE ›› 2014, Vol. 35 ›› Issue (8): 85-88.doi: 10.7506/spkx1002-6630-201408016

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A Real-Time PCR to Detect Horse and Donkey Ingredients in Donkey Hide Glue

WU Ya-jun, WANG Bin, LIU Ming-chang, HAN Jian-xun, LI Xin-shi, CHEN Ying*

Agro-product Research Center, Chinese Academy of Inspection and Quarantine, Beijing 100123, China

Received:2013-07-23 Revised:2014-04-09 Online:2014-04-25 Published:2014-05-13
Contact: CHEN Ying E-mail:liumch30224@126.com

Abstract

Abstract:

In this study, we established a highly specific and sensitive real-time polymerase chain reaction (PCR) method to
detect horse and donkey components in donkey hide glue. The mitochondrial tRNA-Thr gene of horse and the mitochondrial
D-loop region of donkey were selected as target sequences for the designing of specific primers. In conventional and realtime
PCR assays, horse and donkey ingredients could be specifically detected using both primer pairs.

Key words: donkey hide glue, horse, donkey, real-time polymerase chain reaction (PCR)

CLC Number:

R282.5

WU Ya-jun, WANG Bin, LIU Ming-chang, HAN Jian-xun, LI Xin-shi, CHEN Ying*. A Real-Time PCR to Detect Horse and Donkey Ingredients in Donkey Hide Glue[J]. FOOD SCIENCE, 2014, 35(8): 85-88.

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A Real-Time PCR to Detect Horse and Donkey Ingredients in Donkey Hide Glue

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