Abstract:

The fatty acid contents in cold-pressed and hot-pressed perilla oils were determined by reversed phase high
performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD) in a gradient elution mode
after conversion of the triglycerides into free fatty acids by alkaline hydrolysis. The results showed that the differences in
the contents of fatty acids in cold-pressed and ho-pressed perilla oils were highly significant. The contents of linolenic and
linoleic acids in cold-pressed perilla oil were significantly higher, whereas the contents of oleic acid and palmitic acid were
relatively lower than in hot-pressed oil. The content of five fatty acids accounted for up to 95.09% of total fatty acids in coldpressed
oil, but only 87.89% in hot-squeezed oil. The recoveries of five fatty acids were 99.70%–101.11%, with relative
standard derivations (RSDs) between 0.44% and 3.66%.

Key words: reversed phase high performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD), perilla oil, cold-pressed oil, hot-pressed oil, fatty acid composition