Abstract:

Aims: To our knowledge, the production of aureofuscin is very low in the wild-type strain of Streptomyces
aureofuscus (S. aureofuscus). This study attempted to increase the production of aureofuscin by over-expression of a
regulatory gene in the wild-type strain. Methods and Results: The aurj3M gene was amplified by PCR from S. aureofuscus
SYAU0709, ligated into the vector pMD19, and sequenced. Recombinant bacterial strains were constructed by transforming
SYAU0709 with an expression plasmid (pBJAUM) that contained aurj3M, thereby increasing the number of aurj3M gene
copies. Conclusions: The fermentation results showed aurJ3M gene could promote aureofuscin production. Specifically,
the recombinant strain produced approximately 600% more aureofuscin, as quantified by high-performance liquid
chromatography analysis. The recombinant strain also had good genetic stability.

Key words: Streptomyces aureofuscus, aureofuscin, aurj3M gene, fermentation