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Isolation, Identification and Characterization of Contaminant Bacteria from Pleurotus eryngii

YAO Lu-ye, SHEN Jin-bo, DAI Lu, PEI Dan-dan, SUN Dan-feng, JI Hong   

  1. School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Online:2014-05-15 Published:2014-05-19

Abstract:

This study aimed to identify and characterize the pathogenic bacteria which caused pollution in Pleurotus eryngii.
Two strains named CTE722-B and CTE722-C were isolated from Pleurotus eryngii polluted in summer collected from a
mushroom factory. According to their microstructure, physiological, biochemical properties and 16S rDNA sequences,
the phylogenetic positions of the two strains were defined. By measuring the diameter of inhibition zone, the efficacy of
sanitizers commonly used in the food industry against them was investigated. The results showed that CTE722-B and
CTE722-C were identified as Pseudomonas putida and Pseudomonas marginais, respectively. Although the growth of two
strains was restrained within a short time after adding dichloroisocyanuric acid sodium salt, cresol soap or benzalkonium
bromide into the medium, the antibacterial efficacy of these sanitizers was poor due to the high volatility.

Key words: Pleurotus eryngii, pathogenic bacteria, Pseudomonas, characteristics