Advances in the DPPH Radical Scavenging Assay for Antioxidant Activity Evaluation

doi:10.7506/spkx1002-6630-201409062

Abstract

Abstract:

in vitro evaluation of the antioxidant activity of plant compounds or plant extracts is an important aspect of
functional factor research. The DPPH (1-diphenyl-2-picrylhydrazyl) radical scavenging assay is widely used for antioxidant
activity evaluation of plant compounds and extracts. However, this method lacks a standardized program so that results from
different experiments may be difficult to compare with each other. The present review presents a comprehensive overview
of the principle, measurement procedure and wavelength, initial DPPH free radical concentration, reaction time, calculation
of the scavenging rate, expression of results and evaluation indices. Several external factors influencing the DPPH assay are
also discussed.

Key words: DPPH method, free radical, antioxidant, antioxidant activity

WEI Xian-ya, YIN Li-qin, ZHONG Cheng, ZHANG Ming-hai, NIU Ying-ze. Advances in the DPPH Radical Scavenging Assay for Antioxidant Activity Evaluation[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201409062.

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Advances in the DPPH Radical Scavenging Assay for Antioxidant Activity Evaluation

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