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Optimization of Ultrasonic-Assisted Enzymatic Extraction of Anthocyanins from Purple Sweet Potato by Response Surface Methodology

ZHANG Man1, PAN Li-jun1,2,*, JIANG Shao-tong1,2, MO Yu-wen1   

  1. 1. College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China;
    2. Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei 230009, China
  • Online:2014-05-25 Published:2014-05-29
  • Contact: PAN Li-jun

Abstract:

The ultrasonic-assisted enzymatic extraction of anthocyanins from purple sweet potato was optimized by Box-
Behnken experimental design and response surface methodology to increase the yield of anthocyanins. The optimum
extraction conditions were obtained as follows: in a solvent made up of 0.1% hydrochloride and ethanol (50:50, V/V) at
51 ℃, hydrolysis with 54 U/mL cellulase for 33 min at a ultrasonic power of 100 W. The yield of anthocyanins was up to
(3.581 ± 0.016)‰ under these conditions. Compared with the traditional solvent extraction method, the extraction duration
was shortened and the yield of anthocyanins was increased by 2.73 folds using this method, and by 32.4% and 17.8%,
respectively, compared with microwave-assisted method and ultrasonic-assisted method.

Key words: purple sweet potato, anthocyanins, cellulose, ultrasonic, response surface methodology

CLC Number: