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Optimization of Fermentation Conditions for Enhanced 1-Deoxynojirimycin Content in Fermented Mulberry Leaf Tea by Response Surface Methodology

XIAO Hong1, HUANG Xian-zhi2, SHEN Yi-hong2, ,*, QIN Ying-rui1, ZENG Yi-tao1, YANG Juan1, DING Shun-jie1, SHANG Sang1   

  1. 1. Chongqing Key Laboratory of Agricultural Product Processing, College of Food Science, Southwest University,
    Chongqing 400715, China; 2. State Key Laboratory of Silkworm Genome Biology, Chongqing 400715, China
  • Online:2014-05-25 Published:2014-05-29
  • Contact: DING Xiao-wen

Abstract:

Response surface methodology was used to optimize the fermentation conditions for producing fermented
mulberry leaf tea with higher 1-deoxynojirimycin (1-DNJ) content. The 1-DNJ content was investigated with respect to
fermentation temperature, fermentation time and inoculum amount by response surface regression analysis. The optimum
fermentation conditions that provided a 1-DNJ content of 133.882 mg/100 g dry weight of fermented mulberry leaf tea were
determined as 30 ℃, 5.6 h, a ratio of Aspergillus niger to Rhizopus japonicus Vuillemin to Trichoderma viride of 2:1:2, and
an inoculum amount of 3.75×107 CFU/100 g.

Key words: fermentation, mulberry leaves, 1-deoxynojirimycin (1-DNJ), response surface analysis

CLC Number: