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Decolorization and Monosaccharide Composition Analysis of Polysaccharides from Inonotus obliguus

XUAN Guang-shan, LI Qing, WANG Yan-bo   

  1. Qingdao University of Science & Technology, Qingdao 266042, China
  • Online:2014-05-25 Published:2014-05-29

Abstract:

In the present work, activated carbon powder, H2O2, chitosan, and polyamide column chromatography were
compared for their effects in decolorizing Inonotus obliguus polysaccharides, and the decolorized polysaccharides were
analyzed for monosaccharide composition by high performance liquid chromatography (HPLC) after derivatization with
1-pheny-3-methyl-5-pyrazolone. All decolorants were able to decolorize Inonotus obliguus polysaccharides, with activated
carbon powder and polyamide column chromatography being more significantly effective than the other decolorants. The
decolorization efficiency of polyamide column chromatography was 89.3% while retaining 91.7% of polysaccharides. The
decolorized polysaccharides were mainly composed of mannose, rhamnose, glucose, galactose, xylose, and arabinose with a
molar ratio of 2.13:1.36:7.01:2.98:1:1.78.

Key words: Inonotus obliguus, polysaccharides, decolorization, precolumn derivatization, component analysis

CLC Number: