Abstract:

A chromatographic fingerprint analysis method for Australian Virgara Shiraz wine was established by high
performance liquid chromatography-diode array detector-ion trap mass spectrometry (HPLC-DAD-MS). Samples were
extracted with acetic ether, concentrated with a rotary evaporator, and re-dissolved with methanol. HPLC-MS separation was
performed on a MG Ⅱ C18 column (150 mm × 2.0 mm, 5 μm) by gradient elution using a mobile phase composed of water
and acetonitrile adjusted to pH 3.0 at a flow rate of 0.25 mL/min, and multi-wavelength detection was used. The ion trap
mass spectrometry was operated in the positive ion mode with scan range m/z 50 to 1 200 for qualitative and quantitative
analysis of gallic acid and 11 other compounds. A good separation of 15 common peaks of Virgara Shiraz wine was achieved
within 60 min, and 6 of them were confirmed by comparison with reference substances, and the similarity among 10 batches
of Virgara Shiraz wines was all higher than 0.9. The developed fingerprint method is simple, sensitive, stable, accurate, and
suitable for the quality evaluation of Virgara Shiraz wine.

Key words: high perfomance liquid chromatography-diode array detector-ion trap mass spectromtry (HPLC-DAD-MS);chromatographic fingerprint, similarity, import wine, quality evaluation