Total Flavonoids Determined by HPLC in Extracts of Gynura bicolor DC.

doi:10.7506/spkx1002-6630-201412032

Abstract

Abstract:

Six samples collected at different stages during the separation of total flavonoids from crude extracts of the fresh
stems and leaves of Gynura bicolor DC. were analyzed for total flavonoid contents. After undergoing acid hydrolysis, the crude
extracts were determined for quercetin and kaempferol by high-performance liquid chromatography (HPLC). The amount of
total flavonoids was calculated based on the measured flavonoid compounds. After sequential column chromatographies on
macroporous resin and polyamide, the total flavonoid content of purified products was 84.8%, and further recrystallization formed
two products containing 97.1% and 99.7% total flavoniods, respectively. In conclusion, the process involving ethanol extraction,
separation by sequential column chromatographies on macroporous resin and polyamide and recrystallization offers an effective
way to purify total flavonoids from the fresh stems and leaves of Gynura bicolor DC.

Key words: Gynura bicolor DC., high-performance liquid chromatography (HPLC), total flavonoids, quercetin, kaempferol

CLC Number:

Q819

REN Bing-ru, Lü Han, CHEN Jian, LIANG Cheng-yuan, WU Ju-lan, LI Wei-lin*. Total Flavonoids Determined by HPLC in Extracts of Gynura bicolor DC.[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201412032.

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Total Flavonoids Determined by HPLC in Extracts of Gynura bicolor DC.

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