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Optimization of Preparation of Catechin-Loaded Folate-Conjugated Chitosan Nanoparticles by Response Surface Analysis

LIU Bu-yun1, WANG Yong-li2, YU Qi-jian1, LIU Fang1, ZANG Jin-hong1, JIN Ling-he1, LI Da-peng1, LI Feng1,*   

  1. 1. College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China;
    2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2014-07-25 Published:2014-08-26
  • Contact: LI Feng

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Abstract:

The preparation process for catechin-loaded folate-conjugated chitosan nanoparticles (Catechin-FA-CS) was
optimized using response surface methodology. Folate chitosan conjugates (FA-CS) were synthesized based on chemical
linking of carboxylic group of active folate ester with amino group of chitosan. Then, Catechin-FA-CS nanoparticles were
prepared by self-assembling. The optimum process conditions were as follows: mass ratio of catechin to FA-CS 9:20,
mass ratio of FA-CS to sodium tripolyphosphate 3.93:1, pH 5.15, and stirring time 32.35 min. Under these conditions, the
experimental catachin entrapment efficiency was 23.45%, which was close to the predicted value of 23.90%. Transmission
electron micrograph showed that catechin-FA-CS particles were well dispersed as a near-spherical shape within the range of
200–500 nm. These results showed that response surface methodology is applicable for the optimization of preparation of
Catechin-FA-CS nanoparticles.

Key words: catechin, folic acid, chitosan, nanoparticles, response surface analysis

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