Abstract:

Sodium alginate (AL) and chitosan (CH) were coated onto liposome through layer by layer self-assembly
technique. The physicochemical and morphological characteristics and in vitro digestive stability of AL-CH-L were observed.
The results showed that 0.6% CH and 0.5% AL were the best choice to prepare AL-CH-L. Thus, the AL-CH-L revealed a
size of (330.6 ± 37.3) nm with a centralized distribution and a zeta potential of (－15.8 ± 0.7) mV, and a polymer film was
successfully coated on the surface of liposome as observed under a transmittance electron microscope (TEM). Meanwhile,
an obvious core-shell structure was observed. The in vitro digestion experiment carried out using the fluorescence marker
calcein showed better digestive stability of AL-CH-L when compared with common nanoliposomes.

Key words: double modified liposome, chitosan, alginate, layer by layer self-assembly, digestive stability