Abstract:

This study was aimed to establish the optimum conditions for the aqueous enzymatic extraction of papaya seed
oil by investigating the effects of enzymes, hydrolysis time, substrate to water ratio, enzyme dosage and temperature on oil
yield by using single-factor and orthogonal array designs. Meanwhile, the effects of temperature, light and antioxidants on
the oxidative stability of papaya seed oil as reflected by its peroxide value were also analyzed. The results showed that the
optimum parameters for extracting papaya seed oil were found to be 5 h of hydrolysis using neutral protease at a dosage of
2.5% with a substrate to water ratio of 1:7 (g/mL) at 45 ℃. Under the optimized conditions, the extraction yield of papaya
seeds oil was 85.73%. Temperature, light and oxygen caused an increase in peroxide value during storage of papaya seed oil
and TBHQ had good antioxidant effect on the oil.

Key words: aqueous enzymatic method, papaya seed oil, extraction, oxidative stability