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Microwave Extraction Assisted with the Surfactant Sodium Dodecyl Sulphate and Antioxidant Activity Evaluation of Total Flavonoids from Fingered Citron (Citrus medica var. sarcodactylis)

ZHAO Li-li1, CAI Lu-xi2, YE Jing-xiong2, FENG Jie2, ZHAO Yu-ling2,*   

  1. 1. Zhejiang Zhongding Detect Technology Co. Ltd., Yiwu 322000, China;
    2. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
  • Online:2014-09-25 Published:2014-09-17
  • Contact: ZHAO Yu-ling

Abstract:

In the present study, the impacts of SDS concentration, material-to-liquid ratio, microwave power, and irradiation
time on the extraction of total flavonoids from the fruits of fingered citron (Citrus medica var. sarcodactylis) were explored
by single factor and orthogonal array designs. Results indicated that the optimal extraction conditions were determined as
follows: SDS concentration, 0.6%; material-to-liquid ratio, 1:20 (g/mL); microwave power, 50 W; and irradiation time, 30 s.
The yield of total flavonoids under the optimized conditions was 18.706 0 mg/g. The total flavonoids scavenged 79.15% of
DPPH radical and 65.25% of ABTS radical, showing a slightly weaker radical scavenging activity than quercetin. Therefore,
the total flavonoids from fingered citron have potent antioxidant activity and can be used as antioxidants in the food and
medicine fields.

Key words: total flavonoids from fingered citron, SDS, microwave extraction, antioxidant activity

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