Evaluation of the Accuracy of Indirect Competitive ELISA Used to Detect Milk β-Lactoglobulin

doi:10.7506/spkx1002-6630-201418020

FOOD SCIENCE

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Evaluation of the Accuracy of Indirect Competitive ELISA Used to Detect Milk β-Lactoglobulin

YUAN Shui-lin1,2, XIONG Ding1,2, CHEN Hong-bing1,3, GAO Jin-yan2, LI Xin1,2,*

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2. School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330047, China;
3. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China

Online:2014-09-25 Published:2014-09-17
Contact: LI Xin

Abstract

Abstract:

An indirect competitive enzyme-linked immunosorbent assay (ELISA) was established with the standard
β-lactoglobulin as the coated antigen, specific rabbit antibody against β-lactoglobulin as the primary antibody, horseradish
peroxidase labeled goat anti-rabbit IgG:HRP as the secondary antibody and o-phenylenediamine as the substrate solution.
Reversed-phase high-performance liquid chromatography (RP-HPLC) was used as the reference method. The t-test method
proved that the indirect competitive ELISA for detection of β-lactoglobulin was feasible and accurate. The results of t-test
at a significance level α = 0.5 showed that the indirect competitive ELISA was more accurate to detect the concentration of
β-lactoglobulin.

Key words: milk allergy, β-lactoglobulin, indirect competitive enzyme-linked immunosorbent assay, reversed-phase highperformanceliquid chromatography

CLC Number:

TS207.3

YUAN Shui-lin1,2, XIONG Ding1,2, CHEN Hong-bing1,3, GAO Jin-yan2, LI Xin1,2,*. Evaluation of the Accuracy of Indirect Competitive ELISA Used to Detect Milk β-Lactoglobulin[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201418020.

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Evaluation of the Accuracy of Indirect Competitive ELISA Used to Detect Milk β-Lactoglobulin

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