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Identification and Quantitation of Cyclic Adenosine 3’,5’-monophosphate in Seeding Watermelon by Liquid Chromatography-Tandem Mass Spectrometry and High Performance Liquid Chromatography

XI Dong-hua1, GAO Jing2, PENG Xin-yuan1, WANG Qian3, LI Ling1, WU Bin3,*   

  1. 1. College of Chemistry and Chemical Engineering, Xinjiang University, Ürümqi 830046, China;
    2. Center for Physics and Chemistry Analysis, Xinjiang University, Ürümqi 830046, China;
    3. Institute of Agro-products Storage and Processing, Xinjiang Academy of Agricultural Sciences, Ürümqi 830091, China
  • Online:2014-09-25 Published:2014-09-17
  • Contact: WU Bin

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Abstract:

A method to identify cAMP using liquid chromatography coupled to tandem mass spectrometry (LC-MSMS)
and to quantify the content of cAMP in seeding watermelon using high performance liquid chromatography (HPLC)
was established. The quantitative method showed a good linear relationship in the cAMP concentration range from 0.1 to
100 mg/L with a correlation coefficient of 0.999 9. The average recovery for spiked samples was 98.40% with relative
standard deviation (RSD) of 3.22% (n = 5). By using one-factor-at-a-time and orthogonal array designs, the optimum
conditions for cAMP extraction were determined as follows: solid-to-water ratio; 1:12 (fresh sample) or 1:60 (dry sample);
temperature, 90 ℃; extraction time, 40 min. This method is simple, accurate, reliable and reproducible, and can be used for
the determination of cAMP in seeding watermelon.

Key words: seeding watermelon, cyclic adenosine 3’,5’-monophosphate (cAMP), liquid chromatography coupled to tandem
mass spectrometry (LC-MS-MS), high performance liquid chromatography (HPLC)

CLC Number: