Purification and Characteristics of an Aflatoxin B1-Degrading Enzyme from Pseudomonas stutzeri F4

doi:10.7506/spkx1002-6630-201419034

Abstract

Abstract:

Aflatoxin B1 (AFB1)-degrading enzyme (ADE) was purified from Pseudomonas stutzeri F4 by a three-step
procedure including ammonium sulfate fractional precipitation and chromatography on DEAE-Sepharose and Sephadex
G-100. A total of 123-fold purification of ADE with a recovery of 56% and an ultimate specific activity of 6.4 × 104 U/mg
were achieved. The apparent molecular mass of ADE was estimated to be about 24 kD by SDS-PAGE. The ADE displayed
the highest degradation activity for AFB1 at 30 ℃ and pH 6.0. Cu2+ exhibited strong inhibitory activity with an apparent Km
of 5.61 × 10-5 mol/L as indicated by the double-reciprocal plot.

Key words: Pseudomonas stutzeri F4, aflatoxin B1(AFB1)-degrading enzyme, purification, characteristics

CLC Number:

Q814.1

YANG Wen-hua, LI Hai-xing, LIU Xiao-hua, CAO Yu-sheng. Purification and Characteristics of an Aflatoxin B1-Degrading Enzyme from Pseudomonas stutzeri F4[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201419034.

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Purification and Characteristics of an Aflatoxin B1-Degrading Enzyme from Pseudomonas stutzeri F4

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