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Determination of Papaverine and Other Alkaloids in Hot Pot Soup Seasoning by HPLC-MS-MS

YANG Jie, WANG Zhi, ZHANG Hui-zhen*, ZHAI Shi-xing, YU Zhou, ZHANG Feng-yan, YIN Ji-yu, WANG Xiao-bin   

  1. Qingdao Supervision and Testing Center of Product Quality, Qingdao 266061, China
  • Online:2014-11-25 Published:2014-12-09

Abstract:

A method for the determination of five alkaloid residues in hot pot soup seasoning using a high performance liquid
chromatography-tandem mass spectrometry (HPLC-MS-MS) was described. The pretreatment procedure, chromatographic
conditions and mass spectrometric conditions were optimized. The detection limits for morphine, codeine, thebaine, narcotine
and papaverine were 2.4, 2.4, 1.2, 0.048 and 0.024 ng/mL and the quantitative determination limits were 8.0, 8.0, 4.0, 0.16
and 0.08 ng/mL, respectively. The spiked recoveries were between 81.3% and 103.5%, and the relative standard deviations
ranged from 3.02% to 4.55%. This method has the advantages of easy operation, high sensitivity and accuracy.

Key words: papaveirne, liquid chromatography-tandem mass spectrometry, multi-reaction monitoring (MRM)

CLC Number: