Abstract:

1-Deoxynojirimycin (DNJ) has efficient hypoglycemic activity and huge health and medicinal potential.
Therefore, the study of its stability is very meaningful. In this study, the effects of six factors including microwave,
ultrasonic, acid and alkali, heat, ultraviolet light and natural light on the stability of DNJ in Ramulus mori extract were
explored, and the contents were measured by high performance liquid chromatography (HPLC). The results showed that
microwave could destabilize DNJ obviously, and DNJ loss rate was 65% when treating it for 1 h with a power of 800 W.
Yet ultrasound had little effect on DNJ stability, and the content of DNJ in the extract was increased at a certain time point
during the treatment under various powers. Heating had an observable influence on the stability of DNJ, but the effects of pH
and light on the stability of DNJ were weak. The results can provide effective references for the production and storage of
natural DNJ.

Key words: 1-deoxynojirimycin (DNJ), Ramulus mori extract, stability, high performance liquid chromatography