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Establishment of A Method for Detecting Fluorescence Labeled Lycium barbarum Polysaccharide in Rat Plasma

CHEN Chen1,2, TANG Huali1,3, MA Yue1, WANG Shaokang1, YANG Ligang1, PENG Jing4, SUN Guiju1,*   

  1. 1. Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Nutrition and Food Hygiene,
    School of Public Health, Southeast University, Nanjing 210009, China; 2. Wuxi Institute of Arts and Technology, Wuxi 214200,
    China; 3. College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, China;
    4. Tourism Institute, Yangzhou University, Yangzhou 225000, China
  • Online:2015-01-25 Published:2015-01-16
  • Contact: SUN Guiju

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Abstract:

A rapid and sensitive fluorescent method was developed to determine fluorescein isothiocyanate-labeled Lycium
barbarum polysaccharides (LBP-FITC) in rat plasma. The linear calibration curve was obtained in the range from 0.05
to 10 μg/mL in rat plasma and the linear equation was y = 1 378.7x-14.98 (R2 = 0.998 9). The inter-day coefficients of
variation (CVs) at 0.1, 1.0 and 10 μg/mL of LBP-FITC were between 1.97% and 6.90%, and the intraday CVs at the three
concentrations were between 2.62% and 4.15%. The recovery rates of LBP-FITC in rat plasma were between 93.37% and
95.72%. LBP-FITC was stable in rat plasma. The established method is suitable for LBP-FITC pharmacokinetic study. After
oral administration of LBP-FITC to rats, the non-compartmental model was used to calculate the main pharmacokinetic
parameters by WinNonlin software. The results of pharmacokinetic parameters indicated that the peak concentration (Cmax)
was (2.39 ± 0.71) μg/mL, the time to reach the peak value (tmax) was (1.17 ± 0.41) h, the area under the curve (AUC0-t) was
(45.85 ± 7.12) (μg·h)/mL, the AUC0-∞ was (127.31 ± 39.00) (μg·h)/mL, the t1/2 was (80.32 ± 32.70) h, the clearance (CL) was
(1 693.96 ± 492.47) mL/kg, and the mean retention time (MRT) was (20.64 ± 1.36) h.

Key words: fluorescein isothiocyanate-labeled Lycium barbarum polysaccharides (LBP-FITC), pharmacokinetics, stability, recovery rate

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