Abstract:

An ultra performance liquid chromatography-isotope dilution mass spectrometry (UPLC-IDMS) method has
been developed for the determination of biotin in milk powder. After spiking biotin-D2 as an internal standard, samples were
extracted with water. The mobile phase consisted of 10 mmol/L ammonium acetate with 0.1% formic acid and acetonitrile.
The biotin was identified under multiple-reaction monitoring (MRM) mode and quantified by an internal standard method.
The results showed a good linear calibration in the range of 1–50 ng/mL, the recoveries at three spiked levels and the relative
standard deviations were 89.6%–93.1% and 1.71%–4.33%, respectively. The limit of detection and quantification for biotin
were 0.6 μg/100 g and 1.5 μg/100 g, respectively. This method is sensitive, time saving, and accurate for the determination
of biotin in milk powder.

Key words: biotin, ultra performance liquid chromatography-isotope dilution mass spectrometry (UPLC-IDMS), milk powder