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Synergism of Glucosamine Sulfate in Relieving Effect of Hyaluronic Acid by Oral Administration on Mice with Adjuvant Arthritis

HANG Xingwei1, YASUNOBU Masuda2, MAMORU Kimura2, MIKA Usuda2, WANG Wei2, CHEN Jie1, ZHANG Xiaoan1, XI Yinci1,*   

  1. 1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
    2. Japan Kewpie Corporation, Tokyo 1500002, Japan
  • Online:2015-03-15 Published:2015-03-17

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Abstract:

Aim: To explore the synergism of glucosamine sulfate (GS) with hyaluronic acid (HA) by oral administration in
relieving adjuvant arthritis in mice with. Methods: Fifty 8-week-old ICR male mice were randomly divided into 5 groups
at random with an equal number of animals in each group: normal, model, HA, GS, and HA + GS groups. Except normal
group, the animal models with adjuvant arthritis from 4 other groups were established by injecting 0.1 mL of Freund’s
complete adjuvant (FCA) into the left rear paw, followed by oral administration of HA and/or GS for 4 weeks. The body
weights and swelling degree of mouse rear feet were evaluated respectively. The serum concentration of IL-1 and TNF-α
in mice were detected by enzymelinked immunosorbent assay (ELISA). Results: Swelling degree of mouse feet could be
inhibited by oral administration of HA or GS, and this inhibitory effect was more pronounced when both compounds were
administrated. In addition, an obvious decrease in IL-1 and TNF-α levels in serum was observed. Conclusion: In addition to
decreasing serum IL-1 and TNF-α levels, HA and GS could alleviate the swelling of mouse feet more effectively and rapidly
when used in combination than when used individually, so that exerting a better curative effect on mouse arthritis.

Key words: oral administration of hyaluronic acid, oral administration of glucosamine sulfate, adjuvant arthritis

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