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Optimization of Saccharification Conditions of Taro Pulp by Central Composite Design

DAI Yuanyuan, PAN Lijun*, JIANG Shaotong, WU Xuefeng, YU Zhenyu   

  1. Key Laboratory for Agriculture Processing Product of Anhui Province, Institute of Food Science and Engineering,
    School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Online:2015-03-25 Published:2015-03-17

Abstract:

In order to increase the content of reducing sugar in taro pulp, one-factor-at-a-time experiments and central
composite design method were used to optimize the saccharification conditions of the pretreated and liquefied taro pulp.
A quadratic regression model was proposed by response surface analysis. The optimal saccharification conditions were
established as follows: glucoamylase dosage, 170 U/g; temperature, 55 ℃; hydrolysis time, 125 min; and pH, 4.7. Under
these conditions, the dextrose equivalent value of taro pulp reached (21.37 ± 0.06)%, which was in the 95% prediction
interval of the response value. It was suggested that the regression equation had a good prediction capability. Therefore, the
optimal process may be applied to the preparation of high-quality fermented drinks and clarified juice beverages from taro.

Key words: taro pulp, saccharification, dextrose equivalent value, central composite design

CLC Number: