Abstract:

In order to find optimal conditions for the extraction of pyrazine compounds formed by Maillard reaction by using
headspace solid-phase microextraction (HS-SPME), the appropriate fiber was selected and the effects of electrolyte (NaCl)
concentration, temperature and time on extraction efficiency were investigated by single factor and orthogonal array design
methods. The best extraction efficiency was achieved by addition of 1.4 g of the electrolyte to 7 mL of the Maillard reaction
solution and extraction proceeded for 30 min using 75 μm CAR/PDMS fiber at 50 ℃. The relative standard deviation (RSD)
of pyrazine compounds extracted by the proposed procedure ranged from 0.31% to 5.03% with an average of 1.90%. The
method has a good repeatability and can be used to extract pyrazine compounds formed by Maillard reaction.

Key words: headspace solid-phase microextraction (HS-SPME), orthogonal array design, pyrazine compounds, Maillard reaction, gas chromatography