Analysis of Lactic Acid Bacteria in Kefir Starter by 16S rDNA PCR-RFLP

doi:10.7506/spkx1002-6630-201507029

Abstract

Abstract:

Lactic acid bacteria in kefir starter cultures were analyzed and identified to obtain strains with outstanding genetic
traits and excellent fermentation characteristics. A total of 67 lactic acid bacteria strains were obtained directly from kefir
starter cultures by isolation and streak plating. All of these stains could be divided into 6 different groups by conventional
microbiological analysis. Then 4 genotypes were classified by RFLP analysis of the 16S rDNA region, including L. lactis,
E. durans, E. italicus and S. thermophilus. E. durans was the dominant species accounting for 62.7% of all the isolates.

Key words: kefir starter cultures, lactic acid bacteria, 16S rDNA PCR-RFLP

CLC Number:

TS201.3

LI Jia1, LI Yan1,2, MOU Dehua1,*. Analysis of Lactic Acid Bacteria in Kefir Starter by 16S rDNA PCR-RFLP[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201507029.

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Analysis of Lactic Acid Bacteria in Kefir Starter by 16S rDNA PCR-RFLP

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