Abstract:

A large amount of red lotus peel waste, which contains abundant nutrients, is produced during mechanical peeling
of red lotus seeds. Accordingly, insufficient utilization of this byproduct is a serious waste of resource. The present study
examined the impact of various factors on the extraction efficiency of protein from lotus seed peel waste by the combined use of
Plackett-Burman design, steepest ascent design and response surface methodology. We found that 0.03 mol/L Na2CO3-NaHCO3
buffer solution at pH 10.5 was the best solvent for the extraction of lotus seed peel waste protein. A solvent-to-solid ratio of 17.5:1,
an extraction temperature of 20 ℃ and 1 h extraction proved optimal, resulting in an extraction yield of 93.38%.

Key words: lotus seed, peel waste, protein, response surface analysis