FOOD SCIENCE

• Basic Research •     Next Articles

Effect of Recombinant Lipoxygenase on Rheological Properties of Wheat Dough and Bread Quality

REN Di, XIE Yajuan, LU Zhaoxin, ZHANG Chong, BIE Xiaomei, ZHAO Haizhen, LÜ Fengxia*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2015-07-15 Published:2015-07-08

Abstract:

The interaction between arabinoxylan and wheat gluten is essential for rheological properties of dough and wholewheat
bread. In this paper, free thiol content, surface hydrophobicity and viscoelasticity of wheat gluten after enzymatic
action were measured to investigate the effect of recombinant lipoxygenase (ana-rLOX) on the interaction between watersoluble
arabinoxylan (WEAX) and wheat gluten. Meanwhile, further exploration of gluten rheology and bread quality was
conducted by adding recombinant lipoxygenase and soluble arabinoxylan. The results showed that the addition of anarLOX
and WEAX could decrease free thiol content by 21%, increase gluten protein surface hydrophobicity and enlarge
hydrodynamic radius by 27.3%. At the same time, the viscoelasticity of gluten was significantly enhanced and bread quality
was remarkably improved as indicated by reduced harness, gumminess, chewiness and increased resilience.

Key words: recombinant lipoxygenase, water-soluble arabinoxylan, wheat gluten, viscoelasticity, bread quality

CLC Number: