Abstract:

Molecular biology techniques can provide direct authenticity and quality identification of wines. The resolution of
technical problems on grape DNA extraction from wines and the subsequent application of molecular marker techniques in
grapevine identification are of great interest among researchers and of high practical value. A literature review on molecular
biology techniques used for grapevine identification for wines in recent decades is given in this paper, including grape DNA
extraction from wines, DNA quality evaluation and grapevine identification. A systematic comparison of DNA extraction
and downstream detection protocols reported in the literature is provided as well as a detailed analysis of the three steps
involved in DNA extraction namely DNA enrichment, crude DNA extraction and subsequent removal of polyphenol and
polysaccharide, and DNA purification. The technical advances in grapevine identification represented by microsatellite
marker technology are displayed. Meanwhile, the application prospects of gene detection technologies in authenticity and
quality identification of wines are discussed in this paper.

Key words: wine, DNA extraction, microsatellite marker, grapevine identification