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Analysis of Characteristic Aroma Compounds in Wangzhihe Stinky Tofu by Simultaneous Distillation-Extraction and Gas Chromatography-Mass Spectrometry (SDE-GC-MS)

SUN Jiewen, YANG Keyu, LI Yanmin, CHEN Yiying, LIU Yuping, ZHANG Yuyu*   

  1. Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry,
    Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China
  • Online:2015-08-25 Published:2015-08-17
  • Contact: ZHANG Yuyu

Abstract:

The characteristic aroma components of Beijing Wangzhihe stinky tofu were extracted by simultaneous
distillation-extraction (SDE), analyzed by gas chromatography-mass spectrometry (GC-MS) and quantified by internal
standard method. Based on the calculated retention indices and RI qualitative analysis, 44 compounds were identified. These
compounds included 6 alcohols (10.43 μg/g), 17 esters (6.88 μg/g), 1 phenol (0.26 μg/g), 3 carbonyl compounds (0.15 μg/g),
5 sulfur-containing ethers (1.01 μg/g), 6 heterocyclic compounds (2.67 μg/g), and 6 other compounds (2.66 μg/g). The major
aroma components were 1-butanol (7.21 μg/g), 1-propanol (2.33 μg/g), ethyl acetate (1.71 μg/g), butyl butyrate (1.44 μg/g),
and butyl acetate (1.13 μg/g). Indole, 1-butanol, 1-propanol, dimethyl disulfide, dimethyl trisulfide and dimethyl tetrasulfide
made a greater contribution to odor characteristics of Wangzhihe stinky tofu.

Key words: stinky tofu, simultaneous distillation-extraction (SDE), characteristic aroma components, gas chromatography-mass spectrometry (GC-MS), odor active values (OAV)

CLC Number: