Abstract:

The total flavonoids and triterpenoids of loquat flower extracts were purified with D101, AB-8 and polyamide
resins and dried by atmospheric drying, vacuum drying, freeze drying and spray drying, respectively. The effectiveness
of these methods was tested based on the content and yield of total flavonoids and triterpenoids. The results showed that
AB-8 was the best macroporous resin for the separation and purification of flavonoids and triterpenoids, and vacuum drying
was the best drying method. The optimal conditions for purification were determined as follows: sample volume, 2.1 mL;
elution flow rate, 2 BV/h; adsorption time, 4 h; and eluent volume, 3 BV. Under these conditions, the content and yield of
flavonoids were 74.82% and 76.56% and the content and yield of triterpenoids were 15.57% and 46.48%, respectively. The
optimal conditions for vacuum drying were obtained by 10 h drying at 45 ℃ with a vacuum degree of 0.07 MPa. Under the
optimized drying conditions, flavonoids content was 76.07% with a yield of 77.87%, triterpenoids content was 15.89% with
a yield of 47.09% and moisture content was 4.07%.

Key words: loquat flowers, flavonoids, triterpenoids, separation and purification, drying