Assessment of Heavy Metal Pollution by Expression of Yak Metallothionein Gene in Qinghai

doi:10.7506/spkx1002-6630-201518032

Abstract

Abstract:

In order to study the potential risk of heavy metal contamination in Qinghai, total RNA was extracted from yak
liver samples from five regions, Yushu, Guoluo, Haiyan, Gangcha and Gonghe, and then cDNA was generated under the
action of reverse transcription polymerase. The product of reverse transcription reaction was finally used to carry out RTPCR
and detect the expression levels of metallothionein (MT) gene. The results showed that when yak liver sample from
Gangcha was taken as the control group and the value of 2-ΔΔCt was set to be 1, the fluorescence quantitative values of yak
livers from Yushu, Guoluo, Haiyan and Gonghe were 0.669, 0.624, 0.529 and 1.320, respectively. Meanwhile, these results
were confirmed by comparison with those of ELISA reaction. The exposure levels of heavy metal in yak body from different
regions in Qinghai were evaluated, so that we could come up with assessment to yak production safety. Both the results of
gene expression (mRNA level) and ELISA reaction (protein level) displayed that the most serious pollution regions of heavy
metal were Gonghe and Gangcha.

Key words: metallothionein, real-time polymerase chain reaction, gene expression, yak, enzyme-linked immuno sorbent assay

CLC Number:

TS201.2

XUE Yanfeng, LUO Yihao, LIU Xiangjun, XIE Fenglian, JIANG Guobin, SUN Wancheng. Assessment of Heavy Metal Pollution by Expression of Yak Metallothionein Gene in Qinghai[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201518032.

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Assessment of Heavy Metal Pollution by Expression of Yak Metallothionein Gene in Qinghai

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