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Uncertainty Evaluation for Determination of Methyltestosterone Residue in Aquatic Products by High Performance Liquid Chromatography

ZHANG Huifeng1, QI Tianhua2   

  1. Changchun Inspection Center for Aquatic Products Safety and Quality, Changchun 130033, China
  • Online:2015-09-25 Published:2015-09-11

Abstract:

According to the basic methods for uncertainty evaluation from Evaluation and Expression of Uncertainty
in Measurement (JJF1059-2012) and Guidance on Evaluating the Uncertainty in Chemical Analysis (CNAS-GL-06) a
mathematical model of uncertainty evaluation for the determination of methyltestosterone residue in aquatic products by
HPLC was established. The sources of determination uncertainty were analyzed and each component of uncertainty was
quantified. The combined standard uncertainty and expanded uncertainty were finally obtained. The major uncertainty
sources were the random effects in testing process, curve calibration and sample pretreatment.

Key words: uncertainty, methyltestosterone , high performance liquid chromatography (HPLC), aquatic products

CLC Number: