Abstract:

Crystallization of calcium oxalate in the urinary system is one of the important causes of urinary stones, and
enzymatic degradation of oxalic acid for preventing calcium oxalate stones has become a research hotspot. Under in
vitro experimental conditions, the factors that affect the re-dissolution of calcium oxalate crystals catalyzed by oxalate
decarboxylase were studied. The results indicated that when 4 U of oxalate decarboxylase was added to the 10-mL reaction
system which was subsequently allowed to react at pH 3.0 and 37 ℃ for a week, the re-dissolution rate of calcium oxalate
crystals was approximately 5%. The experimental results showed that the degradation product formate produced an
inhibitory effect on the re-dissolution of calcium oxalate crystals catalyzed by oxalate decarboxylase. This experiment
provides a theoretical reference for the study of enzyme preparation to control calcium oxalate stones.

Key words: oxalate decarboxylase, calcium oxalate, dissolution