Isolation, Identification and 16S rDNA Sequence Analysis of Bacterial Fruit Blotch Pathogen in Melon

doi:10.7506/spkx1002-6630-201519033

Abstract

Abstract:

Twelve fruits of melon with bacterial fruit blotch collected from Changji, Xinjiang were investigated for the
isolation and purification of the pathogen. The pathogenic strain was subjected to polymerase chain reaction (PCR) amplification
with the bacterial 16S rDNA universal primer using its total DNA as the template and the products were cloned into pGM-T
vector. Sequencing analysis revealed that the fragment had a length of 1 493 bp. Basic Local Alignment Search Tool (BLAST) and
phylogenetic tree analysis showed that the strain belongs to Acidovorax avenae subsp. citrulli Willems (Aac).

Key words: melon, bacterial fruit blotch, Acidovorax avenae subsp. citrulli Willems, identification

CLC Number:

Q945.8

WANG Xinhui, LIU Xiaoying, LI Guan. Isolation, Identification and 16S rDNA Sequence Analysis of Bacterial Fruit Blotch Pathogen in Melon[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201519033.

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Isolation, Identification and 16S rDNA Sequence Analysis of Bacterial Fruit Blotch Pathogen in Melon

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