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Determination of Deoxynivalenol in Wheat Grains by HPLC Using Solid-Phase Extraction Cleanup Column

LUO Yingpeng, CHEN Zhengxing, WANG Ren, LUO Xiaohu, LI Yongfu, LI Yanan, SHAO Huili, WANG Li   

  1. National Engineering Laboratory for Cereal Fermentation Technology, State Key Laboratory of Food Science and Technology,
    School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Online:2015-10-25 Published:2015-10-20
  • Contact: WANG Li

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Abstract:

Deoxynivalenol (DON) is a ubiquitous mycotoxin present in cereal grains such as wheat and corn. This mycotoxin
causes huge damage to human and animal health. This study optimized the experimental conditions including extraction
solvent, cleanup column, composition and flow rate of mobile phase for DON detection by high-performance liquid
chromatography (HPLC) after solid-phase extraction (SPE) cleanup. The optimized HPLC method was established when the
extraction was performed using a mixture of acetonitrile (ACN) and water (84:16, V/V) followed by SPE cleanup on a Bond
Elut Mycotoxin column using a mobile phase consisting of ACN and water (6:94, V/V) at a flow rate of 0.9 mL/min. Under
these conditions, the recoveries of DON with this method ranged from 90.12% to 106.25%, with intra- and inter-day relative
standard deviations between 4.51% and 6.12%, respectively. The proposed method is applicable to determine DON in grains
because of its rapidity as well as good accuracy, repeatability and stability.

Key words: deoxynivalenol, wheat, high performance liquid chromatography (HPLC), solid-phase extraction, detection

CLC Number: