Abstract:

A high performance liquid chromatography (HPLC) with online postcolumn photochemical derivatization and
fluorescence detection (FLD) method was established for the determination of 16 sulfonamide residues in chicken. Chicken
samples were extracted with acetonitrile and the extract was defatted, separated by column chromatography, treated by online
photochemical derivatization and detected using a fluorescence detector. The mobile phase was a mixture of 0.3% glacial
acetic acid and methanol solution at a flow rate of 0.7 mL/min. The column temperature was set as 36 ℃, and the excitation
and emission wavelengths were set as 320 and 450 nm, respectively. The 16 sulfonamide residues were successfully
separated and determined under chosen experimental conditions. The calibration curves showed good linearity in the range
of 0.13–67.89 μg/mL (R ≥ 0.994 0). The recovery rates of the 16 sulfonamide residues were in the range of 64.3%–106.6%
with relative standard deviation (RSD) of 0.3%–13.9%.The limits of detection (LOD) for the sulfonamides were 1–
67.4 μg/kg. The established method is sensitive, accurate, rapid, and suitable for the determination of sulfonamide residues
in chicken.

Key words: chicken, sulfonamides, online photochemical derivatization, HPLC-FLD