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Development of CdTe Quantum Dots-Based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol

ZHANG Jinyan, XIONG Yan, LIAO Qiegen, LI Weihong, WANG Donggen, ZHANG Li, LUO Linguang   

  1. Laboratory of Quality and Safety Risk Assessment for Livestock and Poultry Products (Nanchang),
    Ministry of Agriculture, Key Laboratory of Quality and Safety of Agricultural Products, Institute for Quality and Safety and Standards of
    Agricultural Products, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
  • Online:2015-11-25 Published:2015-12-03

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Abstract:

CdTe quantum dots (QDs) were synthesized in aqueous solution by using aercapto acetic acid as the stabilizer,
and then coupled to the polyclonal antibody (Anti-Clenbuterol pAb, Anti-CLE pAb) with N-hydroxysuccinimide (NHS)
and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC). Spectral analysis further showed that the
photoluminescence (PL) intensity and absorbance value of the CdTe-Anti-CLE pAb were enhanced without significant
shifts in the characteristic peaks. Using the CdTe-Anti-CLE pAb, an immunochromatographic test strip was developed
for the rapid detection of clenbuterol (CLE) with a detection limit of 0.5 μg/L. Compared with the results of enzymelinked
immunosorbent assay (ELISA), the lateral flow test strip is very useful and effective as a rapid screening method for
detection of CLE residues.

Key words: hydrothermal synthesis, CdTe quantum dots, clenbuterol, immunochromatographic test strip

CLC Number: