Abstract:

In this study, the total protein profiles of Lactobacillus plantarum FS5-5 with salt tolerance grown in media
with NaCl concentrations of 0, 3, 6, and 9 g/100 mL to the middle logarithmic phase were established and compared by
sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and six different protein bands were selected for
analysis by liquid chromatography-tandem mass spectrometry (AB Sciex 4000 QTRAP®LC/MS/MS). Results showed that
6, 11, 9, 4, 15 and 15 proteins were contained in bands 1, 2, 3, 4, 5 and 6, respectively. Totally 45 proteins were identified.
These proteins could be divided into 4 groups including 25 proteins involved in protein synthesis, 10 proteins associated
with metabolism, 8 proteins associated with nucleotide synthesis, and 2 proteins with unknown functions. The differential
expression of these proteins could lead to bacterial protein synthesis, energy metabolism and DNA replication, and
eventually enable Lactobacillus plantarum FS5-5 to better survive in salt stress environment.

Key words: Lactobacillus plantarum FS5-5, salt stress, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), different proteins