Abstract:

Using 1H NMR-based metabolomic approach combined with metabolomics pathway analysis (MetPA), the
metabolic influence of Shanxi aged vinegar (SAV) on rats was carried out. The results indicated that metabolomic profiles of
SAV-treated rats differed greatly from those of normal controls, with a significant change observed in α-glucose, β-glucose,
lactate, citrate, malic acid, lipid, and hippurate. Four pathways including synthesis and degradation of ketone bodies and
metabolism of starch, sucrose, citrate cycle and methyl butyrate were regarded as the targeted metabolic pathways according
to MetPA. The results suggested that the metabolic alteration induced by Shanxi aged vinegar in rats was mainly involved
in energy metabolism, glucose metabolism and lipid pathways. This study revealed the effect of Shanxi aged vinegar on
metabolomic profiles of rats in a holistic way, which can lay the foundation for assessing anti-fatigue, hypoglycemic and
hypolipidemic functions of Shanxi aged vinegar.

Key words: Shanxi aged vinegar, metabolomics, 1H nuclear magnetic resonance, multivariate statistical analysis, metabolomics pathway analysis