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Protective Effects of Tea Extracts and Nano-EGCG against 6-OHDA-Induced Cell Injury in SH-SY5Y Cells

YAN Jingna1, LUO Liyong1,2, HU Yaqiong1,3, ZENG Liang1,2,*   

  1. 1. College of Food Science, Southwest University, Chongqing 400715, China;
    2. Tea Research Institute, Southwest University, Chongqing 400715, China;
    3. Huangyan Branch of China Minsheng Bank, Taizhou 318020, China
  • Online:2016-01-15 Published:2016-01-15

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Abstract:

Human neuroblastoma cell line SH-SY5Y challenged with 6-hydroxydopamine (6-OHDA) was used as an in
vitro model of Parkinson’s disease (PD), and the cytotoxicity of tea extracts including L-theanine, caffeine, tea polyphenol,
epigallocatechin-3-gallate (EGCG), theaflavins, tea pigment, and two kinds of nano-EGCG on SH-SY5Y cells were
investigated, and their protective effects on 6-OHDA-induced SH-SY5Y cells were compared to evaluate their potentials
for the treatment of PD. The results showed that the cytotoxicity was in the following order: tea polyphenol < L-theanine <
tea pigment < caffeine < theaflavins < EGCG; the protective effects were in the following order: theaflavins > EGCG > tea
polyphenol > tea pigment > caffeine > L-theanine. Theaflavins (0.2 μg/mL) and EGCG (0.9 μg/mL) were more effective than
other tea extracts in preventing SH-SY5Y cells from 6-OHDA-induced damage, restoring cell viability to (99.59 ± 2.24)%
and (95.84 ± 2.50)%, respectively. Compared to EGCG, nano-EGCG had weaker cytotoxicity and stronger protective effect.
Based on our results, tea, as a natural daily drink, has better potential for the treatment of PD.

Key words: Parkinson’s disease (PD), tea extracts, 6-hydroxydopamine (6-OHDA), SH-SY5Y cell, nano-epigallocatechin-3-gallate

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