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Purification and Structural Analysis of Polysaccharides from Fruits of Zizyphus jujube Mill. cv. Hupingzao

ZHANG Yaolei1, HUANG Lixin1,*, ZHANG Caihong1,2, XIE Pujun1, ZHANG Qiong1, DING Shasha1   

  1. 1. Jiangsu Province Key Laboratory of Biomass Energy and Material, Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration, National Engineering Laboratory for Biomass Chemical Utilization, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China; 2. Research Institute of Forestry New Technology,Chinese Academy of Forestry, Beijing 100091, China
  • Online:2016-02-15 Published:2016-02-26

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Abstract:

The polysaccharides (ZJP-2 and ZJP-5) from the fruits of Zizyphus jujube Mill. cv. Hupingzao were purified by
Sepharose CL-6B column chromatography, and the structures of the purified active polysaccharides were studied in this
investigation. The purified polysaccharides (ZJP-2b and ZJP-5a) were homogeneous, and their molecular weights were
89.21 and 61.60 kD, respectively. IR spectral analysis showed that ZJP-2b and ZJP-5a had the characteristic absorption
peaks of polysaccharide, and the main structure was β-pyranose for both polysaccharides. The polysaccharide ZJP-2b was
composed of rhamnose, arabinose, mannose, glucose and galactose with a molar ratio of 32.4:9.5:9.4:14.7:9.7, and ZJP-5a
was composed by rhamnose, arabinose, xylose, mannose and galactose with a molar ratio of 20.2:42.9:2.2:7.5:14.5. At the
concentration of 3.5 mg/mL, hydroxyl radical scavenging rates of ZJP-2b and ZJP-5a were 30.51% and 57.22%, respectively.

Key words: Zizyphus jujube Mill. cv. Hupingzao, active polysaccharides, purification, structure

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