Abstract:

Objective: To investigate the antimicrobial resistance and genetic relationship of Salmonella enteritidis in the
production chain of broiler, and to provide the basis for clinical treatment and strain traceability. Methods: The susceptibility
of 171 Salmonella enteritidis to 13 antimicrobial agents was analyzed by micro-dilution method. The molecular types of 33
strains from different production links associated with resistance were analyzed by ERIC-PCR. The data were analyzed by
SPSS 20.0 software, Gel-Pro analyzer 4.0 and NTSYS pc 2.1 software. Results: The resistance degrees of 171 Salmonella
enteritidis to 13 antimicrobial agents were different. The resistance rate of these strains to ampicillin was the highest and up
to 90.06%, and the resistance rate to ofloxacin was the most sensitive and only 5.26%. The resistance rates of different links
were significantly different (P < 0.01). The multidrug resistance rate was 95.32%, and there were 26 kinds of anti-biograms.
Totally 33 Salmonella enteritidis were divided into 4 (I–IV) different genotypes, and the genetic similarity was 66%–100%,
and type I was the dominant genotype. Anti-biograms between the same genotype were also different; conversely, the
genotypes between the same antibiogram were different. Conclusion: The Salmonella enteritidis in broiler production chain
could generate resistance to many antimicrobial agents, generating many kinds of antibiograms. The Salmonella enteritidis
could be spread horizontally along the production chain. The genotypes of broiler farm were relatively complex. There was
no significant correlation between genotype and resistant phenotype.

Key words: Salmonella enteritidis, broiler, antimicrobial resistance, enterobacterial repetitive intergenic consensuspolymerase chain reaction