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Determination of Diethylstilbestrol in Milk Using Dispersive Liquid-Liquid Microextraction Followed by HPLC

QI Qi1, HUANG Min2, XIE Haihua1   

  1. 1. College of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou 341000, China;
    2. The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China
  • Online:2016-02-25 Published:2016-02-23

Abstract:

A novel method for the determination of diethylstilbestrol in milk using dispersive liquid-liquid microextraction
coupled to high performance liquid chromatography (HPLC) was developed. Important parameters affecting extraction
efficiency, such as extraction solvent type and volume, anti-sticking agent type and volume, pH, salt concentration and
temperature, were discussed. Under the optimal conditions, the established method had high sensitivity, low detection limit
and high stability for the analysis of diethylstilbestrol. Ultrasound-assisted ionic-liquid cold-induced aggregation dispersive
liquid-liquid microextraction could be successfully used for the determination of diethylstilbestrol in milk.

Key words: dispersive liquid-liquid microextraction, milk, diethylstilbestrol, high performance liquid chromatography (HPLC)

CLC Number: