FOOD SCIENCE

• Safety Detection • Previous Articles     Next Articles

Determination of Total Sulfite in Xinjiang Specialty Raisin by Fluorescence Derivatization

WANG Jinxia1,2, LIN Jiangli1, LU Yi2,*, WANG Jide1   

  1. 1. Key Laboratory of Oil and Gas Fine Chemicals Ministry of Education and Xinjiang Uyghur Autonomous Region,
    College of Chemistry and Chemical Engineering, Xinjiang University, Ürümqi 830046, China;
    2. Xinjiang Supervision and Inspection Institute for Product Quality, Ürümqi 830004, China
  • Online:2016-02-25 Published:2016-02-23
  • Contact: LU Yi

RichHTML

2

PDF (PC)

220

Abstract:

A rapid and accurate method for the determination of total sulfite in Xinjiang specialty raisin was developed using
fluorescence derivatization. N-(9-Acridinyl) maleimide (NAM) reacted with the sulfite in raisin to form strong fluorescent
derivatives, which were then detected by fluorometry. The linear range of the method was between 1.0 and 43.2 mg/L, with a
correlation coefficient (R2) of 0.999 3. At three spiked levels, the average recoveries of sulfite in raisin ranged from 92.2% to
100.2% with relative standard deviations lower than 3.80% (n = 3). Furthermore, the results obtained by this method for the
total sulfite content of raisin were consistent with those obtained by the pararosaniline method. These results indicate that the
fluorescence derivatization method can accurately and rapidly determine total sulfite in Xinjiang specialty raisin.

Key words: raisin, total sulfite, fluorescence derivatization

CLC Number: