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Determination of 4-Hydroxyderricin and Xanthoangelol in Different Parts of Angelica keiskei by HPLC-PDA

LI Yali1,2, LUO Yulan2, LIU Jianhua3, WU Jinhong4, ZHONG Yaoguang1,*, WANG Zhengwu4,*   

  1. 1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
    2. Shanghai Institute of Landscape Gardening Science, Shanghai 200232, China;
    3. College of Chemistry and Chemical Engineering, Yibin University, Yibin 644000, China;
    4. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
  • Online:2016-03-25 Published:2016-03-18

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Abstract:

In this study, the contents of 4-hydroxyderricin (4-HD) and xanthoangelol (XAG) in roots, stems and leaves of
Angelica keiskei were determined using high performance liquid chromatography with photo-diode array (HPLC-PDA)
detector. The samples were separated on a Halo C18-column (4.6 mm × 150 mm, 2.7 μm), gradiently eluted with 0.1% formic
acid in water (A) and methanol (B) at a flow rate of 0.3 mL/min and detected at 370 nm. The column temperature was set
at 40 ℃. Good linear relationships between peak area and injection amount of 4-HD and XAG were established within the
range of 0.945–18.900 and 1.048–20.960 μg/mL, respectively. The relative standard deviations (RSDs) of peak area for the
two compounds were 3.13% and 2.52%, respectively, suggesting good reproducibility of the developed method. The average
recoveries of the spiked compounds were 103.70%, and 103.05%, respectively. The results showed that the distribution
of 4-HD in different parts of Angelica keiskei was ranked in decreasing order as follows: stems (149.16 μg/g) > roots
(122.42 μg/g) > leaves (1.24 μg/g), while the order of XAG content was as follows: roots (144.28 μg/g) > stems (75.17 μg/g) >
leaves (1.21 μg/g). The contents of 4-HD and XAG in roots and stems of Angelica keiskei were significantly higher than in leaves.

Key words: Angelica keiskei, 4-hydroxyderricin (4-HD), xanthoangelol (XAG), high performance liquid chromatography with photo-diode array (HPLC-PDA)

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